Chris Ashton has been ruled out of England's match against Australia on Saturday with a calf injury.
Ashton came off the bench to make his first England outing in four years in the victory over South Africa at the beginning of the month, before starting the New Zealand and Japan fixtures and scoring a try against the All Blacks.
But the Sale Sharks wing was replaced due to injury against the Brave Blossoms and is now set to miss two weeks of action.
"Chris came off with an injury on Saturday," said forwards coach Steve Borthwick. "We investigated the injury and unfortunately he's going to miss a couple of weeks, which means he's clearly unavailable for our game this weekend. He'll return home later today.
"It's only a couple of weeks. It's frustrating. I'm disappointed for him, disappointed for us.
"It's great the work he's done in camp over the last few weeks but hopefully he'll be back on the field very soon."
Borthwick gave the squad an otherwise clean bill of health, with Joe Cokanasiga, who scored a try on debut against Japan, working towards full involvement in training.
"Joe Cokanasiga didn't train with the team today, he trained on his own programme, which is just part of the individual management that we have," added Borthwick.
"But he's fully fit and available for the weekend."
Nathan Hughes is back in the squad following the completion of a six-week ban for punching Gloucester flanker Lewis Ludlow and questioning his punishment on social media.
"Nathan's done well since coming back into camp," said Borthwick. "He trained on Friday, trained again Sunday and trained with the team the last couple of days, doing really well. He's in good shape.
"I think he's worked really hard on his conditioning. Clearly you've got to prepare as well as you possibly can in those circumstances.
"You actually make improvements in lots of areas when you have that opportunity to train like that. And I think Nathan's worked really hard in this period he's had off."
12 de diciembre 2018
|Valencia 2 Manchester United 1: Jones scores own goal as Red Devils limp through|
|Solari accepts responsibility for Madrid´s heaviest home defeat in Europe|
|WATCH: Rooney backs Rocky ahead of Canelo showdown|
|McLaren to collaborate with Team Bahrain Merida|
|Las Vegas to host 2020 NFL Draft|
|Stade teenager tragically dies after neck injury|
|Cahill may need to leave Chelsea if he wants to play, admits Sarri|
|Viktoria Plzen 2 Roma 1: Chory winner takes hosts into Europa League|
|Real Madrid 0 CSKA Moscow 3: Champions humiliated at Bernabeu|
|Sarri condemns ´disgusting´ Sterling abuse|
|Hansen hails outstanding Ben Smith ahead of Pau switch|
|Foden, Sterling start for Manchester City v Hoffenheim|
|Sky decision will not affect us in 2019, insist Froome and Thomas|
|Al Ain 3 Team Wellington 3 (aet, 4-3 on penalties): Shootout heartbreak for semi-pros in Club World|
|Jackson to remain Ravens´ starting QB despite Flacco return|
|Rams headline 2019 NFL International Series home teams|
|Rams headline International Series home teams for 2019|
|Atlanta want ´exciting football´ from new boss amid Bielsa links|
|Atlanta still yet to receive ´serious´ bid for ´Bale-like´ Almiron|
|Tottenham v Manchester United to be held at Wembley|
|Matip facing six weeks out with fractured collarbone|
|I´ve had quite a lot of calls – Brailsford encouraged by Team Sky interest|
|Afghanistan Football Federation president provisionally banned by FIFA|
|Knee injury rules Huddersfield´s Mooy out of Asian Cup|
|Arnautovic tipped for Man United move by Austria team-mate Sabitzer|
|Arsenal legend Adams to become RFL president|
|My love for football still burns strong – Toure speaks out amid talk of Premier League return|
|Noble contract extended to 2021|
|Split with Sky is an opportunity, says Brailsford|
|Van der Burgh rolls back the years, two more medals for Hosszu|
|Maitland-Niles praises Sterling for speaking out|
|Life is tough for him right now - Celtic striker Griffiths to take time away from football|
|Froome: Team Sky keen to continue with new partner|
|Emery confirms Koscielny to play against Qarabag|
|Llorente keen on Athletic return|
|I love the guy - Pocock backs under-pressure Cheika|
|Fit-again Ozil in line for Arsenal return against Qarabag|
|Raheem Sterling named Premier League Player of the Month|
|Two more Ligue 1 games postponed due to unrest|
|Pace attack among India´s best ever - Arun|
|Premier League has fostered ´excessive aggression´ among fans, says Scudamore|
|Sharks back Flanagan amid NRL investigation|
|Not good for my recovery! Hoddle delights in Spurs´ Champions League heroics|
|Harris backing Starc to fire in Perth|
|No fears over Atleti´s second-place finish for Simeone|
|Clippers buckled under Raptors pressure - Rivers|
|Icardi: Inter deserved to qualify|
|Team Sky future unclear as Sky opt to end involvement in cycling|
|PSG had to change mentality - Thiago Silva|
|Rockets make change, pick up win over Trail Blazers|
|Everything´s easier when you´ve got Messi – Miranda|
|From George Cross to Arsenal – Montemurro´s rise to make Gunners great again|
|Koulibaly challenges Napoli to win Europa League|
|Raptors star Leonard out against Clippers, may miss matchup with Warriors|
|Butler opens up about heated Timberwolves practice|
|Spurs achieved ´mission impossible´, says Pochettino|
|Dembele stunner pleases Barca boss Valverde|
|Van Dijk lauds ´fantastic´ Alisson|
|Van Dijk´s tackle on Mertens worthy of red card – Ancelotti|
|Klopp ´would have paid double´ for Alisson|
|Klopp: I don´t care about Madrid revenge mission|
|Tension and anxiety got to Inter, laments Spalletti|
|Tuchel hails magnificent PSG as Red Star win seals last-16 spot|
|Mbappe admits frank discussions inspired PSG´s Champions League progress|
|Klopp: Napoli had no answers to outstanding Liverpool|
|Pochettino proud of ´massive, massive achievement´ for Spurs|
11 de diciembre 2018
|Barcelona equal Champions League record|
|Composed and controlled Liverpool see off Napoli to progress|
|Tenerife back to winning ways, AEK beat Hapoel|
|Howedes ´hurt´ after emotional Schalke return ends in defeat|